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Nicotine and metabolites by LCMS using Gemini NX 3u C18 100x2.1mm

Sample Preparation Details

Part No:8B-S029-UBJ
Solid Phase Extraction (SPE) procedure:Note: The solvent volumes shown below are for a 60 mg bed mass. The solvent volumes will need to be adjusted for a smaller or larger bed mass.
Dry:10" Hg for 5 min
Final Prep and Analysis: Inject: 10 µL on HPLC / Tandem Mass Spec (MS-MS) @ (ambient)

Analytes:

Name Spiked
Conc.
Log P pKa % Rec %RSC (n=0)
1 Nornicotine
2 3-Hydroxycotinine
3 Anabasine
4 Cotinine
5 Nicotine

Application Detail (App ID: 20651)

Part No:00D-4453-B0
Dimensions:100 x 2 mm ID
Elution Type:Gradient
Elution A:20mM Ammonium Bicarbonate
Elution B:Acetonitrile
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 90 10
2 3 25 75
3 3.1 90 10
4 5 90 10

Flow Rate:0.5 mL/min
Col. Temp: ambient
Detection: Tandem Mass Spec (MS-MS) @ (22°C)
Detector Info: AB Sciex API 4000™

Analyte Details

Analyte Detail not available

1
Nornicotine
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2
3-Hydroxycotinine
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3
Anabasine
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4
Cotinine
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5
Nicotine
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