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Testosterone and 17-OH-Progesterone by LCMS using Gemini NX 3u C18 50x2.1mm

Sample Preparation Details

Part No:8B-S123-TAK
Solid Phase Extraction (SPE) procedure:Note: The solvent volumes shown below are for a 30 mg bed mass. The solvent volumes will need to be adjusted for a smaller or larger bed mass.
Dry:high vacuum for 2-3 min
Final Prep and Analysis: Inject: 25 µL on HPLC / Tandem Mass Spec (MS-MS) @ (ambient)

Analytes:

Name Spiked
Conc.
Log P pKa % Rec %RSC (n=0)
1 Testosterone
2 17-Hydroxyprogesterone

Application Detail (App ID: 20652)

Part No:00B-4453-B0
Dimensions:50 x 2 mm ID
Elution Type:Gradient
Elution A:Water with 0.1% formic acid
Elution B:Acetonitrile with 0.1% formic acid
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 80 20
2 2.5 25 75
3 4.5 25 75
4 4.6 80 20
5 6 80 20

Flow Rate:0.4 mL/min
Col. Temp: ambient
Detection: Tandem Mass Spec (MS-MS) @ (22°C)
Detector Info: AB Sciex API 4000™

Analyte Details

Analyte Detail not available

1
Testosterone
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2
17-Hydroxyprogesterone
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