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Testosterone and 17-OH-Progesterone by LCMS using Gemini NX 3u C18 50x2.1mm

Sample Preparation Details

Condition 1 :1 mL Methanol
Condition 2 :1 mL Water
Load :Load Sample
Wash 1 :0.6 mL 50% MeOH
Dry :high vacuum for 2-3 min
Elution 1 :0.6 mL 100% MeOH
Elution 2 :0.6 mL 100% MeOH
Evaporate: :Evaporate to dryness
Reconstitute :0 µL initial mobile phase

Application Detail (App ID: 20652)

Part No:00B-4453-B0
Dimensions:50 x 2 mm ID
Elution Type:Gradient
Elution A:Water with 0.1% formic acid
Elution B:Acetonitrile with 0.1% formic acid
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 80 20
2 2.5 25 75
3 4.5 25 75
4 4.6 80 20
5 6 80 20

Flow Rate:0.4 mL/min
Col. Temp: ambient
Detection: Tandem Mass Spec (MS-MS) @ (22°C)
Detector Info: AB Sciex API 4000™

Analyte Details

Analyte Detail not available

1
Testosterone
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2
17-Hydroxyprogesterone
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