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Estradiol and estrone analysis by LCMS using Gemini 3um NX-C18 50x2mm

Application Detail (App ID: 20653)

Part No:00B-4453-B0
Dimensions:50 x 2 mm ID
Elution Type:Gradient
Elution A:H2O + 0.5 mM ammonium hydroxide
Elution B:MeOH + 0.5 mM ammonium hydroxide
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 90 10
2 0.5 90 10
3 2.5 20 80
4 5 10 90
5 5.2 90 10
6 6 90 10

Flow Rate:0.55 mL/min
Col. Temp: ambient
Detection: Tandem Mass Spec (MS-MS) @ (22°C)
Detector Info: AB Sciex API 4000™

Analyte Details

Analyte Detail not available

1
Estrone
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2
Estrone-D4
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3
Estradiol
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4
Estradiol-D3
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5
Estriol
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6
Estriol-D3
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