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Biogenic Amines by LCMS using Kinetex 1.7u C18 50x3.0mm

Sample Preparation Details

Condition 1 :3 mL Methanol at 1 mL/min
Condition 2 :4 mL Water
Load :Load Sample
Wash 1 :3 mL 0.1M HCl
Wash 2 :3 mL Methanol
Elution 1 :3 mL 0.2M Ammonia Solution made up in methanol
Evaporate: :Evaporate to dryness
Reconstitute :0 µL Mobile phase

Application Detail (App ID: 20685)

Part No:00B-4475-Y0
Dimensions:50 x 3 mm ID
Elution Type:Gradient
Elution A:30mM Ammonium formate
Elution B:Acetonitrile
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 80 20
2 3 40 60
3 3.1 0 100
4 4 0 100
5 4.1 80 20
6 5 80 20

Flow Rate:600 µL/min
Col. Temp: ambient
Detection: Tandem Mass Spec (MS-MS) @ (22°C)
Detector Info: AB Sciex API 4000™

Analyte Details

Analyte Detail not available

1
IQx
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2
8-MeIQx
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3
IQ
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4
7,8-DiMeIQx
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5
4,8-DiMeI-Qx
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6
MeIQ
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7
Trp-P-2
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8
Harman
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9
PhIP
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10
Norharman
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11
AaC
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12
MeAaC