Region

Applications

Heterocyclic Amines by LCMS using Kinetex 5u C18 50x2.1mm

Sample Preparation Details

Part No:8B-S029-UBJ
Solid Phase Extraction (SPE) procedure:Note: The solvent volumes shown below are for a 60 mg bed mass. The solvent volumes will need to be adjusted for a smaller or larger bed mass.
Final Prep and Analysis: Inject: 2 µL on HPLC / Tandem Mass Spec (MS-MS) @ (ambient)

Analytes:

Name Spiked
Conc.
Log P pKa % Rec %RSC (n=0)
1 DMIP
2 IQx
3 IQ
4 8-MeIQ
5 4,8-DiMe-IQx
6 7,8-DiMe-IQx
7 Trp-P-2
8 Harman
9 Norharman
10 PhIP
11 AaC
12 MeAaC
13 MeIQ

Application Detail (App ID: 20771)

Part No:00B-4601-AN
Dimensions:50 x 2.1 mm ID
Elution Type:Gradient
Elution A:30 mM Ammonium formate
Elution B:Acetonitrile
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 95 5
2 5 40 60
3 5.1 0 100
4 6 0 100
5 7 95 5

Flow Rate:500 µL/min
Col. Temp: ambient
Detection: Tandem Mass Spec (MS-MS) @ (22°C)
Detector Info: AB Sciex API 4000™

Analyte Details

Analyte Detail not available

1
DMIP

2
IQx
search

3
IQ
search

4
8-MeIQ

5
4,8-DiMe-IQx

6
7,8-DiMe-IQx

7
Trp-P-2
search

8
Harman
search

9
Norharman
search

10
PhIP
search

11
AaC
search

12
MeAaC

13
MeIQ
search