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Heterocyclic Amines by LCMS using Kinetex 5u C18 50x2.1mm

Sample Preparation Details

Condition 1 :3 mL Methanol at 1 mL/min
Condition 2 :4 mL Water
Load :Load Sample
Wash 1 :3 mL 0.1M HCl
Wash 2 :3 mL Methanol
Elution 1 :3 mL 0.2M Ammonia Solution made up in methanol
Evaporate: :Evaporate to dryness
Reconstitute :0 µL Mobile phase

Application Detail (App ID: 20771)

Part No:00B-4601-AN
Dimensions:50 x 2.1 mm ID
Elution Type:Gradient
Elution A:30 mM Ammonium formate
Elution B:Acetonitrile
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 95 5
2 5 40 60
3 5.1 0 100
4 6 0 100
5 7 95 5

Flow Rate:500 µL/min
Col. Temp: ambient
Detection: Tandem Mass Spec (MS-MS) @ (22°C)
Detector Info: AB Sciex API 4000™

Analyte Details

Analyte Detail not available

1
DMIP

2
IQx
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3
IQ
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4
8-MeIQ

5
4,8-DiMe-IQx

6
7,8-DiMe-IQx
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7
Trp-P-2
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8
Harman
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9
Norharman
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10
PhIP
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11
AaC
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12
MeAaC

13
MeIQ
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