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Applications

Testosterone-Oxime on Gemini-NX C18, 3um, 50x2 mm

Sample Preparation Details

Part No:8B-S123-TBJ
Solid Phase Extraction (SPE) procedure:Note: The solvent volumes shown below are for a 30 mg bed mass. The solvent volumes will need to be adjusted for a smaller or larger bed mass.
Final Prep and Analysis: Inject: 25 µL on HPLC / Mass Spectrometer (MS) @ amu (ambient)

Analytes:

Name Spiked
Conc.
Log P pKa % Rec %RSC (n=0)
1 Testosterone
2 Testosterone-D3
3 Epitestosterone

Application Detail (App ID: 20844)

Part No:00B-4453-B0
Dimensions:50 x 2 mm ID
Elution Type:Gradient
Elution A:0.1% Formic Acid plus 1 mM Ammonium Formate in DI H2O
Elution B:0.1% Formic Acid plus 1 mM Ammonium Formate in ACN
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 70 30
2 1.5 10 90
3 2.5 10 90
4 2.6 70 30
5 5 70 30

Flow Rate:0.4 mL/min
Col. Temp: 50 °C
Detection: Mass Spectrometer (MS) @ amu (0°C)
Detector Info: AB Sciex API 5000™

Analyte Details

Analyte Detail not available

1
Testosterone
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2
Testosterone-D3
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3
Epitestosterone
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