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Applications

Testosterone-Oxime on Gemini-NX C18, 3um, 50x2 mm

Sample Preparation Details

Condition 1 :1 mL 100% MeOH at 1 mL/min
Condition 2 :1 mL DI H2O at 1 mL/min
Load :Load 0.25 mL Sample diluted in 1 mL DI H2O
Wash 1 :0.6 mL 50% MeOH at 1 mL/min
Elution 1 :0.6 mL 100% MeOH at 1 mL/min
Evaporate: :Evaporate to dryness
Reconstitute :0 µL initial mobile phase

Application Detail (App ID: 20844)

Part No:00B-4453-B0
Dimensions:50 x 2 mm ID
Elution Type:Gradient
Elution A:0.1% Formic Acid plus 1 mM Ammonium Formate in DI H2O
Elution B:0.1% Formic Acid plus 1 mM Ammonium Formate in ACN
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 70 30
2 1.5 10 90
3 2.5 10 90
4 2.6 70 30
5 5 70 30

Flow Rate:0.4 mL/min
Col. Temp: 50 °C
Detection: Mass Spectrometer (MS) @ amu (0°C)
Detector Info: AB Sciex API 5000™

Analyte Details

Analyte Detail not available

1
Testosterone
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2
Testosterone-D3
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3
Epitestosterone
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