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OPA Derivitization of Histadine and Histamine using Kinetex 2.6u C18

Application Detail (App ID: 20872)

Part No:00D-4462-E0
Dimensions:100 x 4.6 mm ID
Elution Type:Gradient
Elution A:20mM Sodium Acetate pH 7.2 w/0.03% Sodium Azide (pre-filtered 0.2um)
Elution B:50:50 Acetonitrile/Methanol
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 97 3
2 20 20 80
3 20.5 97 3
4 23 97 3

Flow Rate:1.2 
Col. Temp: 30 °C
Detection: UV-Vis Abs.-Variable Wave.(UV) @ 338 nm (22°C)
Detector Info: Offline Amino Acid derivatization:
1. In to a amber vial dispense 250uL of 20mM Sodium Acetate pH 12.5
2. Add 100uL of Sample (ex: Wine sample, Standards)
3. Mix for 30 seconds
4. Add 50uL of OPA derivatization agent (OPA: Part # 5061-3335)
5. Mix vigorously for 30 seconds
6. Add 2.0mL of MilliQ Water
7. Mix for 30 seconds
8. Inject 10uL of sample on to system

Analyte Details

Analyte Detail not available

1
Histidine
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2
Histamine
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