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Melatonin in commercial formuation on Kinetex 5u C18 50x4.6mm

Application Detail (App ID: 20900)

Part No:00B-4601-E0
Dimensions:50 x 4.6 mm ID
Elution Type:Gradient
Elution A:0.25% Phosphoric Acid in MilliQ Water
Elution B:Acetonitrile
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 90 10
2 7 30 70
3 8 10 90
4 10 10 90
5 10.1 90 10
6 13 90 10

Flow Rate:1.5 mL/min
Col. Temp: 30 °C
Detection: UV-Vis Abs.-Variable Wave.(UV) @ 225 nm (22°C)

Analyte Details

Analyte Detail not available

1
Melatonin
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2
Formulation impurity