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Nicotinic acid / Nicotinamide (10 ng/mL) in Human Plasma by Impact on Gemini 3µm C18 100x4.6mm

Sample Preparation Details

SPE Product:Impact™ Protein Precipitation, 2mL Square Well Filter Plate, 2/Pk
Part No:CE0-7565
Solid Phase Extraction (SPE) procedure:Note: The solvent volumes shown below are for a Proprietary bed mass. The solvent volumes will need to be adjusted for a smaller or larger bed mass.
Final Prep and Analysis: Inject: 2 µL on HPLC / Electrospray Mass Spec (ESMS) @ (ambient)

Analytes:

Name Spiked
Conc.
Log P pKa % Rec %RSC (n=0)
1 Nicotinamide 10 
2 Nicotinic acid 10 

Application Detail (App ID: 20906)

Part No:00D-4439-E0
Dimensions:100 x 4.6 mm ID
Elution Type:Gradient
Elution A:0.1% formic acid
Elution B:Methanol 100%
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 90 10
2 2.5 10 90
3 2.6 90 10
4 4 90 10

Flow Rate:0.6 mL/min
Col. Temp: ambient
Detection: Electrospray Mass Spec (ESMS) @ (22°C)
Detector Info: AB Sciex API 4000™

Analyte Details

Analyte Detail not available

1
Nicotinamide
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2
Nicotinic acid
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