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Water-soluble Vitamins on Kinetex 2.6u, C18, 75x4.6 by HPLC (#20715)

Application Detail (App ID: 20908)

Part No:00C-4462-E0
Dimensions:75 x 4.6 mm ID
Elution Type:Gradient
Elution A:3g Sodium 1-hexanesulfonate monohydrate/0.4mL Triethylamine/15mL Acetic acid in 1985mL H2O
Elution B:Methanol
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 95 5
2 6 53 47
3 6.1 2 98
4 7.5 2 98
5 7.51 95 5
6 11 95 5

Flow Rate:0.5 mL/min
Col. Temp: 25 °C
Detection: UV-Vis Abs.-Variable Wave.(UV) @ 270 nm (22°C)

Analyte Details

Analyte Detail not available

1
Ascorbic acid
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2
Pyridoxal 5'-phosphate
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3
Nicotinic acid
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4
4-Pyridoxic acid
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5
Nicotinamide
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6
Riboflavin 5'-phosphate
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7
Pyridoxal
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8
Pyridoxine
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9
Cobalamine

10
Folic acid
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11
Pyridoxamine
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12
Riboflavin
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13
Thiamine
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