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T3/T4 Plasma Extract by LC/MS/MS on Kinetex C18, 2.6 um, 75x2.1 mm

Sample Preparation Details

Part No:8B-S029-UBJ
Solid Phase Extraction (SPE) procedure:Note: The solvent volumes shown below are for a 60 mg bed mass. The solvent volumes will need to be adjusted for a smaller or larger bed mass.
Dry:5 min under max vac
Final Prep and Analysis: Inject: 40 µL on HPLC / Mass Spectrometer (MS) @ amu (ambient)

Analytes:

Name Spiked
Conc.
Log P pKa % Rec %RSC (n=0)
1 T3
2 Rev T3
3 T4

Application Detail (App ID: 20929)

Part No:00C-4462-AN
Dimensions:75 x 2.1 mm ID
Elution Type:Gradient
Elution A:0.05% Formic Acid in DI H2O
Elution B:50:50 MeOH:ACN
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 70 30
2 2.5 30 70
3 3 30 70
4 3.01 10 90
5 4 10 90
6 4.01 70 30
7 5.5 70 30

Flow Rate:600 mL/min
Col. Temp: ambient
Detection: Mass Spectrometer (MS) @ amu (0°C)
Detector Info: AB Sciex API 5000™

Analyte Details

Analyte Detail not available

1
T3
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2
Rev T3

3
T4
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