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Human Plasma Vitamin C on Kinetex 5u XB-C18, 150x4.6mm Recovery-1

Sample Preparation Details

SPE Product:Impact™ Protein Precipitation, 2mL Square Well Filter Plate, 2/Pk
Part No:CE0-7565
Solid Phase Extraction (SPE) procedure:Note: The solvent volumes shown below are for a Proprietary bed mass. The solvent volumes will need to be adjusted for a smaller or larger bed mass.
Final Prep and Analysis: Inject: 1 µL on HPLC / UV-Vis Abs.-Variable Wave.(UV) @ 245 nm (ambient)

Analytes:

Name Spiked
Conc.
Log P pKa % Rec %RSC (n=0)
1 Vitamin C 50 

Application Detail (App ID: 21444)

Part No:00F-4605-E0
Dimensions:150 x 4.6 mm ID
Elution Type:Gradient
Elution A:0.1% formic acid
Elution B:Acetonitrile 100%
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 100 0
2 3.5 100 0
3 3.6 0 100
4 5 0 100
5 5.1 100 0
6 7 100 0

Flow Rate:800 µL/min
Col. Temp: ambient
Detection: UV-Vis Abs.-Variable Wave.(UV) @ 245 nm (22°C)
Note:Guard column and cartridges were used for human plasma vitamin C analysis:
SecurityGuard Ultra Cartridges: Cat log No. AJO-8768.
SecurityGuard Ultra Cartridge holder: Cat log No. AJO-9000.

Analyte Details

Analyte Detail not available

1
Vitamin C
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