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Analysis of Phenylbutazone on Kinetex 2.6u XB-C18 50x2.1 by LC-MS-MS API 4000

Application Detail (App ID: 21871)

Part No:00B-4496-AN
Dimensions:50 x 2.1 mm ID
Elution Type:Gradient
Elution A:0.1% Formic Acid in DI Water
Elution B:0.1% Formic Acid in Methanol
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 50 50
2 0.5 50 50
3 2 5 95
4 3.5 5 95
5 3.51 50 50
6 5 50 50

Flow Rate:0.45 mL/min
Col. Temp: 45 °C
Detection: Mass Spectrometer (MS) @ amu (0°C)
Detector Info: AB Sciex API 4000
Note:Mass spec conditions:
API 4000, ESI TurboIon Spray, + Ionization;
CAD: 6.00
CUR: 20.00
GS1: 50.00
GS2: 50.00
IS: 5500.00
TEM: 600.00
ihe: ON
DP 50.00
EP 10.00


Analyte Details

Analyte Detail not available

1
Phenylbutazone
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2
Phenylbutazone-d10
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3
impurity