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Mass spec conditions:
API 4000, ESI TurboIon Spray, + Ionization;
CAD: 6.00
CUR: 20.00
GS1: 50.00
GS2: 50.00
IS: 5500.00
TEM: 600.00
ihe: ON
DP 50.00
EP 10.00
Extraction Procedure:
1. Condition a Strata-X-A, 100 mg/6 mL with 3 mL 100% MeOH followed by 3 mL DI water
2. Load sample from the final step of pretreatment section
3. Wash with 2 mL DI H2O
4. Wash with 2 mL 100% ACN
5. Wash with 2 mL 100% Ethylacetate
6. Dry the SPE bed for 4-5 min @ max vacuum
7. Elute with 2x1.5 mL 1% formic acid in MeOH
8. Evaporate the eluate to dryness under gentle nitrogen stream at 45-50 C
9. Re-suspend the residue with 500 uL 50:50 MeOH:0.1% formic acid in DI H2O