Region

Applications

Human Plasma TMP & Thiamine by Gemini-NX 3u C18 100x3mm with Impact Plate_STD-TMP

Sample Preparation Details

SPE Product:Impact™ Protein Precipitation, 2mL Square Well Filter Plate, 2/Pk
Part No:CE0-7565
Solid Phase Extraction (SPE) procedure:Note: The solvent volumes shown below are for a Proprietary bed mass. The solvent volumes will need to be adjusted for a smaller or larger bed mass.
Final Prep and Analysis: Inject: 20 µL on HPLC / Fluorescence (FLUOR) @ Ex 375 nm (ambient)

Analytes:

Name Spiked
Conc.
Log P pKa % Rec %RSC (n=0)
1 TMP 200 
2 Thiamine 200 

Application Detail (App ID: 21876)

Part No:00D-4453-Y0
Dimensions:100 x 3 mm ID
Elution Type:Gradient
Elution A:25nM Na2HPO4, 10% methanol
Elution B:25mM Na2HPO4, 70% methanol
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 3 97
2 0.25 25 75
3 0.75 25 75
4 3 35 65
5 4 100 0
6 5 100 0
7 5.1 3 97
8 8 3 97

Flow Rate:0.75 mL/min
Col. Temp: ambient
Detection: Fluorescence (FLUOR) @ Ex 375 nm (22°C)
Detector Info: Shimadzu ELSD

Analyte Details

Analyte Detail not available

1
TMP
search

2
Thiamine
search