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Applications

Anabasine using SPE with Gemini NX-C18, LC-MSMS (Fig.3e)

Sample Preparation Details

Part No:8B-S029-UBJ
Solid Phase Extraction (SPE) procedure:Note: The solvent volumes shown below are for a 60 mg bed mass. The solvent volumes will need to be adjusted for a smaller or larger bed mass.
Dry:> 10" Hg for 5 min to remove residual water
Final Prep and Analysis: Reconstitution Solvent: 500uL Acetonitrile/20mM Ammonium bicarbonate (10:90) Inject: 10 µL on HPLC / Tandem Mass Spec (MS-MS) @ (ambient)

Analytes:

Name Spiked
Conc.
Log P pKa % Rec %RSC (n=0)
1 Nornicotine
2 3-OH-Cotinine
3 Anabasine
4 Cotinine
5 Nicotine

Application Detail (App ID: 22040)

Part No:00B-4453-B0
Dimensions:50 x 2 mm ID
Elution Type:Gradient
Elution A:20mM Ammonium Bicarbonate
Elution B:100% Acetonitrile
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 90 10
2 3 25 75
3 3.1 90 10
4 5 90 10

Flow Rate:500 µL/min
Col. Temp: 25 °C
Detection: Tandem Mass Spec (MS-MS) @ (22°C)
Detector Info: AB Sciex API 4000™

Analyte Details

Analyte Detail not available

1
Nornicotine
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2
3-OH-Cotinine

3
Anabasine
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4
Cotinine
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5
Nicotine
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