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Applications

Prednisolone Chromatogram_LLOQ (10 ng/mL)

Analytes

1Cortisone
2Cortisol
3Prednisone
4Prednisolone

Compound Name Cortisone
CID 222786
Molecular Formula C21H28O5
Molecular Weight 360
No. Hydrogen Bond Acceptors 5
No. Hydrogen Bond Donors 2

Details

LC Conditions (App ID: 22168)

Column:

Kinetex 2.6 µm Biphenyl 100 Å, LC Column 50 x 3.0 mm, Ea

Brand Name:

Kinetex

Part No:

Phase Name:

Biphenyl

Elution Type:

Gradient

Mobile Phase:

A: 10 mM ammonium acetate in water

B: 10 mM ammonium acetate in MeOH

Gradient Profile:

Step No.	Time(min)	%A	%B
1	0.01	60	40
2	0.5	60	40
3	2	10	90
4	3	10	90
5	3.01	60	40
6	5	60	40

Flow Rate:

0.4 mL/min

Temperature:

40°C

Detection:

Tandem Mass Spec (MS-MS) (22°C)

Order Items Used in This Application

00B-4622-Y0

Kinetex 2.6 µm Biphenyl 100 Å, LC Column 50 x 3.0 mm, Ea

Qty:

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