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Nicotines at High pH using Kinetex 5u EVO C18 100x3.0mm

Application Detail (App ID: 22721)

Part No:00D-4633-Y0
Dimensions:100 x 3 mm ID
Elution Type:Gradient
Elution A:20 mM Ammonium bicarbonate pH 8.2
Elution B:Methanol
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 90 10
2 3 10 90
3 3.1 90 10
4 5 90 10
5 5 95 5

Flow Rate:0.75 mL/min
Col. Temp: ambient
Detection: Mass Spectrometer (MS) @ 254 amu (22°C)
Detector Info: AB Sciex API 4000™

Analyte Details

Analyte Detail not available

1
3-OH-Cotinine
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2
Nornicotine
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3
Cotinine
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4
Anabasine
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5
Nicotine
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6