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FMOC derivatized glyphosate on a Gemini 3 µm NX-C18

Application Detail (App ID: 22749)

Part No:00B-4453-B0
Dimensions:50 x 2 mm ID
Elution Type:Gradient
Elution A:5 mM Ammonium bicarbonate pH 9
Elution B:ACN/Methanol (50:50)
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 80 20
2 4 10 90
3 4.1 80 20
4 7 80 20

Flow Rate:0.4 mL/min
Col. Temp: ambient
Detection: Tandem Mass Spec (MS-MS) @ (22°C)
Detector Info: API 5000
Note:Derivatization protocol: In a 2 mL vial, spike 50 µL of 10 µg/mL Glyphosate and AMPA standards into 1 mL of 0.4 M borate buffer. Add 200 µL of 2 mg/mL FMOC in acetone (make fresh daily) and vortex.

Analyte Details

Analyte Detail not available

1
Glyphosate
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2
2-Aminoethylphosphonic acid (AMPA)