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Applications
Oxytocin Extracted Ion Chromatograms, QCL (0.500 ng/mL)
22771
Separation Mode: Reversed Phase
Reversed Phase
PFP(2)
HPLC
Antidiuretic Agents
Clinical
Clinical Research
Therapeutic / Clinical

Applications

Oxytocin Extracted Ion Chromatograms, QCL (0.500 ng/mL)
Analytes
1Oxytocin
2ARG-Vasopressin
Compound Name
Endopituitrina
CID
5771
Molecular Formula
C43H66N12O12S2
Molecular Weight
1007
No. Chiral Centers:
9
No. Hydrogen Bond Acceptors
13
No. Hydrogen Bond Donors
12
Details
LC Conditions (App ID: 22771)
Column:
Brand Name:
Part No:
Phase Name:
PFP(2)
Elution Type:
Gradient
Mobile Phase:
A: 0.1%FA in water
B: 0.1%FA in 50:50 MeOH:Acetonitrile
Gradient Profile:
Step No.Time(min)%A%B
109010
20.29010
30.57030
43.57030
53.513070
643070
74.019010
859010
Flow Rate:
0.45 mL/min
Temperature:
22°C
Detection:
Tandem Mass Spec (MS-MS) (40°C)
Detector Info:
SCIEX API 5000
Sample Preparation Details
Description:
Strata-X-CW 33 µm Polymeric Weak Cation, 30 mg / well, 96-Well Plates, 2/Pk
Part Number:
Condition 1:
Methanol
Condition 2:
Water
Wash 1:
4% H3PO4 in water
Wash 2:
30% MeOH in water
Evaporation:
Evaporate to dryness
Sample Notes:
1
Condition
Methanol
2
Shake
1% TFA in 50:50 MeOH: Acetonitrile
1
Shake
1% TFA in 50:50 MeOH: Acetonitrile
2
Shake
1% TFA in 50:50 MeOH: Acetonitrile
1
Shake
1% TFA in 50:50 MeOH: Acetonitrile
2
Shake
1% TFA in 50:50 MeOH: Acetonitrile

Order Items Used in This Application

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