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ARG-Vasopressin Extracted Ion Chromatograms, QCL (0.500 ng/mL)

Sample Preparation Details

Part No:8E-S035-TGB
Solid Phase Extraction (SPE) procedure:Note: The solvent volumes shown below are for a 30 mg bed mass. The solvent volumes will need to be adjusted for a smaller or larger bed mass.
Dry:3-5 min
Final Prep and Analysis: Inject: 10 µL on HPLC / Tandem Mass Spec (MS-MS) @ (40 °C)

Analytes:

Name Spiked
Conc.
Log P pKa % Rec %RSC (n=0)
1 Oxytocin
2 ARG-Vasopressin

Application Detail (App ID: 22772)

Part No:00B-4447-B0
Dimensions:50 x 2 mm ID
Elution Type:Gradient
Elution A:0.1%FA in water
Elution B:0.1%FA in 50:50 MeOH:Acetonitrile
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 90 10
2 0.2 90 10
3 0.5 70 30
4 3.5 70 30
5 3.51 30 70
6 4 30 70
7 4.01 90 10
8 5 90 10

Flow Rate:0.45 mL/min
Col. Temp: ambient
Detection: Tandem Mass Spec (MS-MS) @ (40°C)
Detector Info: AB Sciex API 5000

Analyte Details

Analyte Detail not available

1
Oxytocin
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2
ARG-Vasopressin
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