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EPA 539 using Kinetex 5u EVO C18 100x3.0mm

Application Detail (App ID: 22780)

Part No:00D-4633-Y0
Dimensions:100 x 3 mm ID
Elution Type:Gradient
Elution A:0.2% ammonium hydroxide
Elution B:Methanol with 0.2% ammonium hydroxide
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 65 35
2 0.6 35 65
3 7.5 35 65
4 8.5 15 85
5 13 15 85
6 13.01 35 65
7 15 35 65

Flow Rate:0.3 mL/min
Col. Temp: 30 °C
Detection: Electrospray Mass Spec (ESMS) @ (40°C)
Detector Info: AB Sciex API 4000™

Analyte Details

Analyte Detail not available

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Estriol
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Equilin
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Estrone
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17-B-Estradiol

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17-a-Ethynylestradiol

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Androstenedione
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Testosterone
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