FOR SECURITY PURPOSES - because Internet Explorer is no longer supported by Microsoft, we suggest that you interact with our secure site through one of our supported browsers - Google Chrome, Firefox, or MS Edge. If you continue to use this website with Internet Explorer you do so at your own risk and you may encounter problems.
Extraction Procedure
Sample pre-treatment: Dilute 200 uL of human plasma with 200 ul of 50 mM sodium phosphate dibasic heptahydrate, pH unadjusted. Vortex briefly (3-5 sec).
Sample loading: Load the sample from pretreatment step abive onto the Novum plate and apply a short and gentle pulse of vacuum (~ 5" of Hg for 5-10 sec) or until the sample has completely entered the media.
Wait for 5 minutes.
Elution: Dispense 900 uL of dichloromethane onto the SLE media and allow the solvent to elute by gravity (~ 5 min elution time) and collect the eluant.
Repeat with another 900 uL of dichloromethane and collect the eluant.
Apply vacuum at 5" of Hg for 20-30 secs to complete the extraction.
NOTE: Prolonged application of vacuum will result in elution of plasma out of the SLE media and into the final extracted solvent.
Dry down: Evaporate the final extract to complete dryness under slow stream of N2 at 40°C
Reconstitute the dry residue in 200 uL of initial mobile phase fortified with cortisol-D4.