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LCMS of Vitamin D epimers on Kinetex 2.6u F5 100x4.6mm

Application Detail (App ID: 22810)

Part No:00D-4723-E0
Dimensions:100 x 4.6 mm ID
Elution Type:Isocratic
Elution A:Water with 0.1% formic acid
Elution B:Methanol with 0.1% formic acid
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 15 85

Flow Rate:0.75 mL/min
Col. Temp: ambient
Detection: Tandem Mass Spec (MS-MS) @ (22°C)

Analyte Details

Analyte Detail not available

1
25-OH Vitamin D3

2
25-OH Vitamin D2

3
3-epi-25-OH Vitamin D3