| 1Timolol |
|---|
Compound Name Timolol |
CID 33624 |
Molecular Formula C13H24N4O3S |
Molecular Weight 316 |
No. Hydrogen Bond Acceptors 7 |
No. Hydrogen Bond Donors 2 |
Applications
Extraction of Timolol from Serum using Strata-X-C 10 mg Plate & Kinetex 2.6u Biphenyl 50x2.1 Column
22982
Separation Mode: Reversed Phase
Reversed Phase
Biphenyl
HPLC
Antihypertensive Agents
Applications
Extraction of Timolol from Serum using Strata-X-C 10 mg Plate & Kinetex 2.6u Biphenyl 50x2.1 Column
Analytes
Details
LC Conditions (App ID: 22982)
Column: | ||||||||||||||||||||||||||||
Brand Name: Kinetex | ||||||||||||||||||||||||||||
Part No: | ||||||||||||||||||||||||||||
Phase Name: Biphenyl | ||||||||||||||||||||||||||||
Elution Type: Gradient | ||||||||||||||||||||||||||||
Mobile Phase: A: 0.1% formic acid in water B: 0.1% formic acid in acetonitrile | ||||||||||||||||||||||||||||
Gradient Profile:
| ||||||||||||||||||||||||||||
Flow Rate: 0.5 mL/min | ||||||||||||||||||||||||||||
Temperature: 25°C | ||||||||||||||||||||||||||||
Detection: Tandem Mass Spec (MS-MS) (22°C) | ||||||||||||||||||||||||||||
Sample Note: Strata X-C 10 mg Plate Protocol
Condition
500 µL methanol
Equilibrate
500uL water
Load
750 µL diluted serum
(375 µL plasma diluted 1:1 with 4% phosphoric acid in water)
Wash1
500 µL 2% formic acid in water
Wash 2
500 µL methanol
Elute
375 µL(3x125 µL) 5% ammonium hydroxide in acetonitrile:methanol(60:40)
Inject
1 µL
Note: Spiked conc. of analyte was 1 ng/mL serum |
Sample Preparation Details
Description: Strata X-C 33 µm Polymeric Strong Cation, 10 mg / well, 96-Well Plates, 2/Pk |
Part Number: |
Pre Treatment Note: No dry down step was performed. 1µL of final eluent was injected. |
Condition 1: Methanol |
Condition 2: Water |
Sample PreTreatment: No dry down step was performed. 1µL of final eluent was injected. |
Wash 1: 2% Formic acid in water |
Wash 2: Methanol |
Evaporation: Evaporate to dryness |
1 Condition Methanol |
2 Shake 5% Ammonium hydroxide in Acetonitrile/Methanol (60 |
1 Condition Methanol |
2 Shake 5% Ammonium hydroxide in Acetonitrile/Methanol (60 |
3 Shake 5% Ammonium hydroxide in Acetonitrile/Methanol (60 |
1 Wash 2% Formic acid in water |
2 Condition Water |
Order Items Used in This Application
00B-4622-AN
Kinetex 2.6 µm Biphenyl 100 Å, LC Column 50 x 2.1 mm, Ea
Qty:
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