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A: 0.1% TFA/2% ACN in Water B: 0.1% TFA/ ACN (10:90)
Gradient Profile:
Step No.
Time(min)
%A
%B
1
0
98
2
2
68
30
70
Flow Rate:
1.5 mL/min
Temperature:
60°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) @ 214 nm (22°C)
Sample Note:
Forced degradation protocol:
Reconstitute 1 mg of cefaclor neat standard with 1.0 mL of 1 M HCl. Heat the solution at 60 ºC for 6 hours then neutralize with 1.0 mL of 1 M NaOH. Refrigerate until ready to inject.