Cefaclor forced degradation on Kinetex 2.6 µm EVO C18

Application Detail (App ID: 22988)

Part No:00D-4725-E0
Dimensions:100 x 4.6 mm ID
Elution Type:Gradient
Elution A:0.1% H3PO4 in Water
Elution B:0.1% H3PO4 in ACN
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 95 5
2 6 60 40

Flow Rate:1.75 mL/min
Col. Temp: ambient
Detection: UV-Vis Abs.-Variable Wave.(UV) @ 254 nm (22°C)
Note:Forced degradation protocol:
Reconstitute 1 mg of cefaclor neat standard with 1.0 mL of 1 M HCl. Heat the solution at 60 ºC for 6 hours then neutralize with 1.0 mL of 1 M NaOH. Refrigerate until ready to inject.

Analyte Details

Analyte Detail not available