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Nicotine & Metabolites on Kinetex 2.6µm Evo C18 100x3mm

Application Detail (App ID: 23006)

Part No:00D-4725-Y0
Dimensions:100 x 3 mm ID
Elution Type:Gradient
Elution A:20mM Ammonium Bicarbonate, pH 8.2
Elution B:Methanol
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 90 10
2 3 10 90
3 5 10 90
4 5.01 90 10
5 6 90 10

Flow Rate:750 µL/min
Col. Temp: 25 °C
Detection: Mass Spectrometer (MS) @ amu (0°C)
Detector Info: AB Sciex API 5000

Analyte Details

Analyte Detail not available

1
3-OH-Cotinine

2
Nornicotine
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3
Cotinine
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4
Anabasine
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5
Nicotine
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