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A: 20 mM ammonium bicarbonate pH 10.0 B: Acetonitrile
Gradient Profile:
Step No.
Time(min)
%A
%B
1
0
95
5
2
5
5
95
Flow Rate:
0.7 mL/min
Temperature:
22°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) @ 254 nm (22°C)
Sample Note:
Forced degradation protocol:
Reconstitute 1 mg of cefaclor neat standard with 1.0 mL of 1 M HCl. Heat the solution at 60 ºC for 6 hours then neutralize with 1.0 mL of 1 M NaOH. Refrigerate until ready to inject.