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Applications
Amino Acid Analysis using OPA on Kinetex 5u EVO C18 150x4.6mm
23092
Separation Mode: Reversed Phase
Reversed Phase
EVO C18
HPLC
Glycine Agents
Pharmaceutical/Biopharmaceutical
Life Science
Therapeutic / Clinical
Biochemical Compound / Nutrient
Therapeutic / Clinical

Applications

Amino Acid Analysis using OPA on Kinetex 5u EVO C18 150x4.6mm
Analytes
1ASP
2GLU
3SER
4HIS
5THR
6GLY
7ARG
8ALA
9TYR
10MET
11VAL
12CYS
13PHE
14ILE
15LEU
16LYS
Compound Name
L-Aspartic Acid
CID
5960
Molecular Formula
C4H7NO4
Molecular Weight
133
No. Hydrogen Bond Acceptors
5
No. Hydrogen Bond Donors
3
Details
LC Conditions (App ID: 23092)
Column:
Brand Name:
Kinetex
Part No:
Phase Name:
EVO C18
Elution Type:
Gradient
Mobile Phase:
A: 20mM Potassium Phosphate pH 7.2
B: 50:50 Acetonitrile:Methanol
Gradient Profile:
Step No.Time(min)%A%B
10973
2204060
323973
Flow Rate:
1 mL/min
Temperature:
30°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) @ 338 nm (22°C)

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