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Bupropion Extracted from Urine using Strata X-CW and Kinetex 5u EVO C18

Sample Preparation Details

Part No:8B-S035-UBJ
Solid Phase Extraction (SPE) procedure:Note: The solvent volumes shown below are for a 60 mg bed mass. The solvent volumes will need to be adjusted for a smaller or larger bed mass.
Dry:5 minutes
Final Prep and Analysis: Inject: 2 µL on HPLC / UV-Vis Abs.-Variable Wave.(UV) @ 254 nm (ambient)

Analytes:

Name Spiked
Conc.
Log P pKa % Rec %RSC (n=0)
1 Bupropion

Application Detail (App ID: 23095)

Part No:00F-4633-E0
Dimensions:150 x 4.6 mm ID
Elution Type:Gradient
Elution A:5mM Ammonium Bicarbonate pH 9.0
Elution B:Methanol
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 50 50
2 10 20 80

Flow Rate:1 mL/min
Col. Temp: ambient
Detection: UV-Vis Abs.-Variable Wave.(UV) @ 254 nm (22°C)

Analyte Details

Analyte Detail not available

1
Bupropion
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