Region

Applications

Bupropion related compounds on Kinetex 5u EVO C18 150x4.6mm

Sample Preparation Details

Condition 1 :3 mL Metahnol at 1 mL/min
Condition 2 :3 mL Water at 1 mL/min
Load :Load 1.00 mL Sample diluted in 100mM Sodium Phosphate pH 6.8
Wash 1 :3 mL 100mM Sodium Phosphate pH 6.8 at 1 mL/min
Dry :5 minutes
Elution 1 :2 mL 5% Formic Acid in Acetonitrile at 0.5 mL/min
Evaporate: :Evaporate to dryness
Reconstitute :500 µL 50:50 5mM Ammonium Bicarbonate pH 9.0 and Methanol

Application Detail (App ID: 23096)

Part No:00F-4633-E0
Dimensions:150 x 4.6 mm ID
Elution Type:Gradient
Elution A:5mM Ammonium Bicarbonate pH 9.0
Elution B:Methanol
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 50 50
2 10 20 80

Flow Rate:1 mL/min
Col. Temp: ambient
Detection: UV-Vis Abs.-Variable Wave.(UV) @ 254 nm (22°C)

Analyte Details

Analyte Detail not available

1
Hydroxybuproprion

2
Erythrobuproprion
search

3
Threobupropion
search

4
Bupropion
search