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Applications
Vitamin A and E on Kinetex EVO C18, APCI Pos/Neg Polarity Switching LC/MS/MS
23286
Separation Mode: Reversed Phase
Reversed Phase
EVO C18
HPLC
Antioxidants

Applications

Vitamin A and E on Kinetex EVO C18, APCI Pos/Neg Polarity Switching LC/MS/MS
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Analytes
1Vitamin A (all-trans-retinol) [Pos Polarity]
2Vitamin E (gamma-tocopherol) [Neg Polarity]
3Vitamin E (a-tocopherol) [Neg Polarity]
Compound Name
Retinol
CID
445354
Molecular Formula
C20H30O
Molecular Weight
286
No. Hydrogen Bond Acceptors
1
No. Hydrogen Bond Donors
1
Details
LC Conditions (App ID: 23286)
Column:
Brand Name:
Kinetex
Part No:
Phase Name:
EVO C18
Elution Type:
Gradient
Mobile Phase:
A: DI H2O
B: 50:50 IPA:ACN
Gradient Profile:
Step No.Time(min)%A%B
103565
23595
34595
44.13565
55.53565
Flow Rate:
0.6 mL/min
Temperature:
22°C
Detection:
Tandem Mass Spec (MS-MS) (1°C)
Detector Info:
API 5000

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