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Vitamin D tablet assay (Naturemade 2000 IU) on a Kinetex 2.6 µm Biphenyl, 150 x 4.6

Application Detail (App ID: 23496)

Part No:00F-4622-E0
Dimensions:150 x 4.6 mm ID
Elution Type:Isocratic
Elution A:0.1% TFA
Elution B:Acetonitrile
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 35 65

Flow Rate:1.75 mL/min
Col. Temp: 40 °C
Detection: UV-Vis Abs.-Diode Array (PDA) @ 280 nm (22°C)
Note:Sample preparation: small tablets (<400 mg)

1. Grind tablet into powder and transfer to a 2 mL dSPE tube (KS0-8916)
2. Spike in 20 µL of triphenylene internal standard (1 mg/mL) and standard additions (if applicable)
3. Add 800 µL of water
4. Sonicate until dissolved (approx. 5 min)
5. Add 800 µL of acetonitrile
6. Shake for 10 min using mechanical shaker
7. Centrifuge at 15000 rpm for 3 min
8. Aspirate 100 µL of supernatant and transfer to an autosampler vial for analysis

Sample preparation: large tablets (>400 mg)

1. Grind tablet into powder and transfer to a 5 mL centrifuge tube
2. Spike in 20 µL of triphenylene internal standard (1 mg/mL) and standard additions (if applicable)
3. Add 2 mL of water
4. Sonicate until dissolved (approx. 5 min)
5. Add 400 mg of Quechers salt (AH0-9044)
6. Add 2 mL of acetonitrile
7. Shake for 10 min using mechanical shaker
8. Centrifuge at 6000 rpm for 3 min
9. Aspirate 100 µL of supernatant and transfer to an autosampler vial for analysis

Analyte Details

Analyte Detail not available

1
Triphenylene (I.S.)

2
Vitamin D3
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