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Nicotine metabolites using Luna Omega Polar C18 50x2.1mm

Application Detail (App ID: 23594)

Part No:00B-4748-AN
Dimensions:50 x 2.1 mm ID
Elution Type:Gradient
Elution A:10mM ammonium formate with 0.1% formic acid
Elution B:Acetonitrile with 0.1% formic acid
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 98 2
2 3 10 90
3 3.1 98 2

Flow Rate:400 mL/min
Col. Temp: 25 °C
Detection: Tandem Mass Spec (MS-MS) @ (450°C)
Detector Info: AB Sciex API 4000™

Analyte Details

Analyte Detail not available

1
Nornicotine
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2
3-Hydroxycotinine
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3
Nicotine
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4
Cotinine
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5
Anabasine
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