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THC-COOH comparison using Beta-Gone and Dilute-n-Shoot

Sample Preparation Details

Part No:8B-S133-TAK
Solid Phase Extraction (SPE) procedure:Note: The solvent volumes shown below are for a 30 mg bed mass. The solvent volumes will need to be adjusted for a smaller or larger bed mass.
Load:To 200 µL urine, add 133 µL of 0.1% Formic acid in Methanol.
Pass through the Beta-Gone tube or 96-well plate and collect eluent.
Final Prep and Analysis: Inject: 10 µL on HPLC / Tandem Mass Spec (MS-MS) @ (ambient)

Analytes:

Name Spiked
Conc.
Log P pKa % Rec %RSC (n=0)
1 THC-COOH

Application Detail (App ID: 23627)

Part No:00B-4462-AN
Dimensions:50 x 2.1 mm ID
Elution Type:Gradient
Elution A:Water with 0.1% Formic acid
Elution B:Acetonitrile with 0.1% formic acid
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 95 5
2 3 5 95
3 4 5 95
4 4.1 95 5

Flow Rate:500 µL/min
Col. Temp: ambient
Detection: Tandem Mass Spec (MS-MS) @ (22°C)
Detector Info: AB Sciex API 4000™

To 200 µL urine, add 133 µL of 0.1% Formic acid in Methanol. Pass through the Beta-Gone tube or 96-well plate and collect eluent.

Analyte Details

Analyte Detail not available

1
THC-COOH
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