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FAMEs In Peanut Butter by GC/FID on Zebron ZB-FAME

Application Detail (App ID: 23825)

Part No:7HG-G033-10
Phase:Proprietary Pesticides Phase
Dimensions:30 meters x 0.25 mm x 0.2 µm
Oven Profile:100 ºC for 2 min to 140 ºC @ 10 °C/min to 190 ºC @ 3 ºC/min to 260 ºC @ 30ºC/min for 2 min
Carrier Gas:Constant Flow Helium, 1.2 mL/min
Injection:Split 50:1 1 µL @ 240°C
Detection: Flame Ionization (FID) (260°C)
Note:Recommended Liner: Zebron PLUS Single Taper with Wool, 4 mm ID
Liner Part No.: AG2-0A11-05 (for Agilent systems)
Inlet Seal: AG0-8620 (Gold Plated Easy Seal)
Septum: AG0-4696 (PhenoRed-400)

Sample Preparation:
1. Weigh out 100 to 200 mg of peanut butter and place it into a scintillation vial
2. Add 100 mg pyrogallic acid
3. Add 2 mL ethanol followed by 10 mL 8.3 M HCl
4. Incubate at 70 °C for 45 min
5. Extract using 2 mL diethyl ether and 2 mL chloroform
6. Blow down extraction liquid
7. Reconstitute in 1 mL toluene and 2 mL 8 % boron trifluoride in methanol
8. Cap reaction mixture, hold @ 100 °C for 45 min
9. After reaction, add 5 mL water, 1 mL hexane, and 1 g Na2SO4
10. Extract hexane layer for GC analysis

Analyte Details

Analyte Detail not available

1
C11:0 (I.S.)

2
C14:0
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3
C16:0
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4
C17:0
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5
C18:0
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6
C18:1 trans 9
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7
C18:1 cis 9
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8
C18:2 cis 9,12
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9
C20:0
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10
C20:2 cis 11,14
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11
C22:0
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12
C24:0
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