| 1C8:0 |
|---|
| 2C10:0 |
| 3C11:0 (I.S.) |
| 4C12:0 |
| 5C14:0 |
| 6C16:0 |
| 7C18:0 |
| 8C18:1 cis 9 |
| 9C18:2 cis 9,12 |
| 10C18:3 cis 9,12,15 |
| 11C20:0 |
| 12C20:1 cis 11 |
| 13C20:4 cis 5,8,11,14 |
| 14C22:0 |
| 15C24:0 |
| 16C22:6 cis 4,7,10,13,16,19 |
Compound Name Methyl Octanoate |
CID 8091 |
Molecular Formula C9H18O2 |
Molecular Weight 158 |
No. Hydrogen Bond Acceptors 2 |
Column: |
Part No: |
Phase Name: ZB-FAME |
Injection: Split 50:1 @ 240°C, 1.0 μL |
Oven Program: 100 ºC for 2 min to 140 ºC @ 10 °C/min to 190 ºC @ 3 ºC/min to 260 ºC @ 30 ºC/min for 2 min |
Carrier Gas: Constant Flow Helium, 1.2 mL/min |
Detection: Flame Ionization (FID) (260°C) |
Sample Notes: Recommended Liner: Zebron PLUS Single Taper with Wool, 4 mm ID
Liner Part No.: AG2-0A11-05 (for Agilent systems)
Inlet Seal: AG0-8620 (Gold Plated Easy Seal)
Septum: AG0-4696 (PhenoRed-400)
Sample Preparation:
1. Weigh out 500 mg of powdered infant formula into a scintillation vial
2. Dissolve (or dilute) sample in 5 mL toluene
3. Add 6 mL 10 % acetyl chloride solution in methanol
4. Incubate @ 80 °C for 2 hours
5. After incubation add 10 mL 10 % Na2CO3 solution and centrifuge at 5000 rpm for 5 min
6. Extract organic layer for GC analysis |
