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FAMEs In Infant Formula by GC/FID on Zebron ZB-FAME

Application Detail (App ID: 23827)

Part No:7HG-G033-10
Phase:Proprietary Pesticides Phase
Dimensions:30 meters x 0.25 mm x 0.2 µm
Oven Profile:100 ºC for 2 min to 140 ºC @ 10 °C/min to 190 ºC @ 3 ºC/min to 260 ºC @ 30 ºC/min for 2 min
Carrier Gas:Constant Flow Helium, 1.2 mL/min
Injection:Split 50:1 1 µL @ 240°C
Detection: Flame Ionization (FID) (260°C)
Note:Recommended Liner: Zebron PLUS Single Taper with Wool, 4 mm ID
Liner Part No.: AG2-0A11-05 (for Agilent systems)
Inlet Seal: AG0-8620 (Gold Plated Easy Seal)
Septum: AG0-4696 (PhenoRed-400)

Sample Preparation:
1. Weigh out 500 mg of powdered infant formula into a scintillation vial
2. Dissolve (or dilute) sample in 5 mL toluene
3. Add 6 mL 10 % acetyl chloride solution in methanol
4. Incubate @ 80 °C for 2 hours
5. After incubation add 10 mL 10 % Na2CO3 solution and centrifuge at 5000 rpm for 5 min
6. Extract organic layer for GC analysis

Analyte Details

Analyte Detail not available

1
C8:0
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2
C10:0
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3
C11:0 (I.S.)

4
C12:0
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5
C14:0
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6
C16:0
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7
C18:0
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8
C18:1 cis 9
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9
C18:2 cis 9,12
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10
C18:3 cis 9,12,15
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11
C20:0
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12
C20:1 cis 11
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13
C20:4 cis 5,8,11,14
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14
C22:0
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15
C24:0
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16
C22:6 cis 4,7,10,13,16,19
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