Region

Applications

EtG / EtS from Urine on Luna Omega 5u Polar C18, 50x4.6mm

Sample Preparation Details

Part No:8B-S038-TAK
Solid Phase Extraction (SPE) procedure:Note: The solvent volumes shown below are for a 30 mg bed mass. The solvent volumes will need to be adjusted for a smaller or larger bed mass.
Load:1. Into individually labeled 1.5 mL conical micro-centrifuge tubes, combine 0.5 mL of 0.1% acetic acid, 50 µL IS, and 100 µL blank, standard, or sample.
2. Activate the SPE cartridge (Strata-X-AW, 30 mg/1mL) with 1 mL pure methanol.
3. Equilibrate the SPE cartridge with 1 mL of 0.1% acetic acid.
4. Load sample and proceed with elution.
5. Wash the SPE cartridge with 0.5 mL 50% methanol.
6. Dry the SPE bed under high vacuum for 5-10 min.
7. Elute the analyte with 2 x 0.600 mL 2% ammonium hydroxide in methanol.
8. Evaporate the tubes in a concentrator at 45-50°C.
9. Remove the tubes and resuspend the residue in 200 µL Mobile Phase A (0.1% formic acid).
Dry:5-10 min under high vacuum
Final Prep and Analysis: Inject: 10 µL on HPLC / Tandem Mass Spec (MS-MS) @ (550 °C)

Analytes:

Name Spiked
Conc.
Log P pKa % Rec %RSC (n=0)
1 Interference
2 Ethyl Sulfate (EtS)
3 Ethyl Glucuronide (EtG)

Application Detail (App ID: 23842)

Part No:00B-4754-E0
Dimensions:50 x 4.6 mm ID
Elution Type:Gradient
Elution A:0.1% Formic Acid in H2O
Elution B:0.1% Formic Acid in MeOH
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 98 2
2 2 40 60
3 2.01 10 90
4 3 10 90
5 3.01 98 2
6 4 98 2

Flow Rate:1 mL/min
Col. Temp: 25 °C
Detection: Tandem Mass Spec (MS-MS) @ (550°C)
Detector Info: AB Sciex 4000 QTRAP®
Note:MMA (117.0-->72.9; 117.0-->54.9)
MMA-D3 (120.1-->75.9)

Analyte Details

Analyte Detail not available

1
Interference

2
Ethyl Sulfate (EtS)

3
Ethyl Glucuronide (EtG)