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EtG / EtS from Urine on Luna Omega 5u Polar C18, 50x4.6mm

Sample Preparation Details

Condition 1 :1 mL Methanol
Condition 2 :1 mL 0.1% Acetic Acid in Water
Load :Load 100.00 µL Sample diluted in 0.1% Acetic Acid in Water
Wash 1 :0.5 mL 50:50 Methanol:Water
Dry :5-10 min under high vacuum
Elution 1 :0.6 mL 2% Ammonium Hydroxide in Methanol
Elution 2 :0.6 mL 2% Ammonium Hydroxide in Methanol
Evaporate: :Evaporate to dryness
Reconstitute :200 µL Mobile Phase A

Application Detail (App ID: 23842)

Part No:00B-4754-E0
Dimensions:50 x 4.6 mm ID
Elution Type:Gradient
Elution A:0.1% Formic Acid in H2O
Elution B:0.1% Formic Acid in MeOH
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 98 2
2 2 40 60
3 2.01 10 90
4 3 10 90
5 3.01 98 2
6 4 98 2

Flow Rate:1 mL/min
Col. Temp: 25 °C
Detection: Tandem Mass Spec (MS-MS) @ (550°C)
Detector Info: AB Sciex 4000 QTRAP®
Note:MMA (117.0-->72.9; 117.0-->54.9)
MMA-D3 (120.1-->75.9)

Analyte Details

Analyte Detail not available

1
Interference

2
Ethyl Sulfate (EtS)
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3
Ethyl Glucuronide (EtG)
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