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100 °C for 2 min to 140 °C @ 10 °C/min to 190 °C @ 3 °C/min to 260 °C @ 30 °C/min for 2 min
Carrier Gas:
Constant Flow Helium, 1.2 mL/min
Detection:
Flame Ionization (FID) (260°C)
Sample Notes:
Recommended Liner: Zebron PLUS Single Taper with Wool, 4 mm ID
Liner Part No.: AG2-0A11-05 (for Agilent systems)
Inlet Seal: AG0-8620 (Gold Plated Easy Seal)
Septum: AG0-4696 (PhenoRed-400)
Sample Preparation:
1. Strata® Si-1 Tube, 1 g/6 mL (Part No.: 8B-S012-JCH) on a vacuum or positive pressure manifold
2. Wash cartridge with 6 mL of hexane
3. Load oil solution (0.12 g of oil in 0.5 mL of hexane)
4. Elute with 10 mL of hexane/diethyl ether (87:13)
5. Evaporate eluate under a steady stream of nitrogen
6. Dissolve purified oil residue in 1mL of heptane
7. Add 0.1 mL of 2N potassium hydroxide in methanol to purified oil solution
8. Cap tube and shake vigorously for 15 seconds
9. Leave to separate until upper layer becomes clear
10. Extract upper layer for GC analysis
To reconstituted sample, add 0.1 mL of 2 N Potassium hydroxide in methanol. Cap tube and shake vigorously for 15 seconds. Leave to separate until upper layer becomes clear. Extract upper layer for GC anlaysis.