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THCs extracted from Human Urine using Strata Screen C on GC/MS (TIC)

Sample Preparation Details

Condition 1 :3 mL Methanol at 1 mL/min
Condition 2 :3 mL DI Water at 1 mL/min
Condition 3 :1 mL 20mM Sodium Acetate pH 3.0 at 1 mL/min
Load :Load 2.00 mL Sample diluted in 100uL 10N Sodium Hydroxide + 1mL glacial acetic
Wash 1 :2 mL DI Water at 1 mL/min
Wash 2 :2 mL 100mM HCl/Acetonitrile (95:5) at 1 mL/min
Wash 3 :0.2 mL Hexane at 0.5 mL/min
Dry :5 minutes 10 inches Hg
Elution 1 :3 mL Hexane:Ethyl Acetate (50:50) at 1 mL/min
Evaporate: :Evaporate to dryness
Reconstitute :100 µL Ethyl Acetate/BSTFA
Notes :Add 50uL Ethyl Acete and 50uL BSTFA (with 1% TMCS), Mix/vortex, react 20 minutes @ 70C, remove from heat source and transfer to autosampler vial.

Application Detail (App ID: 23876)

Part No:7CD-G018-08
Phase:5% Phenyl 95% Dimethylpolysiloxane
Dimensions:10 meters x 0.18 mm x 0.18 µm
Oven Profile:200 °C to 300 °C @ 20 °C/min
Carrier Gas:Constant Flow Helium, 0.7 mL/min
Injection:Split 10:1 1 µL @ 280°C
Detection: Mass Spectrometer (MS) (320°C)

Analyte Details

Analyte Detail not available

1
Delta 9 THC
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2
Delta 9 THC-D3

3
Hydroxy THC

4
Hydroxy THC-D3

5
Carboxy THC
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6
Carboxy THC-D3
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