Region

Applications

Benzodiazepines extracted using Strata Screen C 150mg/3cc from Urine (TIC)

Sample Preparation Details

Condition 1 :3 mL Methanol at 1 mL/min
Condition 2 :3 mL 100mM Potassium Phosphate pH 6.0 at 1 mL/min
Load :Load 4.00 mL Sample diluted in Diluted Urine
Wash 1 :2 mL 100mM Potassium Phosphate pH 6.0 at 1 mL/min
Wash 2 :3 mL 20:80 (100mM Potassium Phosphate pH 6.0 : Methanol) at 1 mL/min
Wash 3 :2 mL Hexane at 0.5 mL/min
Dry :Dry for 5 mins at 15" Hg
Elution 1 :3 mL Ethyl Acetate:Isopropanol:Ammonium Hydroxide (70:20:10) at 0.5 mL/min
Evaporate: :Evaporate to dryness
Reconstitute :150 µL Ethyl Acetate (100uL) + MTBSTFA (50uL)
Notes :Reconstitute in 100uL Ethyl Acetate and 50uL MTBSTFA; Cap and incubate @ 60C for 20minutes then transfer to amber vial with insert. Inject 1uL.

Application Detail (App ID: 23893)

Part No:7CD-G018-08
Phase:5% Phenyl 95% Dimethylpolysiloxane
Dimensions:10 meters x 0.18 mm x 0.18 µm
Oven Profile:200 to 340 over 25C/min hold for 1 minute
Carrier Gas:Constant Flow Helium, 17.2 mL/min
Injection:Split 20:1 1 µL @ 260°C
Detection: Mass Spectrometer (MS) (320°C)

Analyte Details

Analyte Detail not available

1
Diazepam
search

2
D5-Nordiazepam

3
Nordiazepam
search

4
D5-Oxazepam

5
Oxazepam
search

6
D5-Temazepam

7
Temazepam
search

8
Clonazepam
search

9
Lorazepam
search

10
Alprazopam

11
D5-alpha-Hydroxyalprazolam

12
Alpha-Hydroxyalprazolam
search