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Barbiturates from urine by Strata X (60mg/3cc) (TIC)

Sample Preparation Details

Condition 1 :3 mL Methanol at 1 mL/min
Condition 2 :3 mL Water at 1 mL/min
Condition 3 :1 mL 20mM potassium phosphate pH 2.7 at 1 mL/min
Load :Load 2.00 mL Sample diluted in Diluted Urine
Wash 1 :3 mL Water at 1 mL/min
Wash 2 :3 mL 100mM acetic acid at 1 mL/min
Dry :Dry column (5 minutes at > 10 inches Hg)
Elution 1 :3 mL 85:15 Ethyl Acetate:Isopropyl Alcholol at 0.5 mL/min
Evaporate: :Evaporate to dryness
Reconstitute :150 µL Acetonitrile/Thermo Scientific MethElute Reagent
Notes :Reconstitute with 100uL Acetonitrile and 50uL of Thermo Scientific MethElute Reagent (TS49301)

Application Detail (App ID: 23895)

Part No:7CD-G023-08
Phase:Proprietary Drug Phase
Dimensions:10 meters x 0.18 mm x 0.18 µm
Oven Profile:145 °C to 300 °C @ 25 °C/min
Carrier Gas:Constant Flow Helium, 0.6 mL/min
Injection:Split 10:1 1 µL @ 280°C
Detection: Mass Selective (MSD) (320°C)
Note:Analytes are 300ng/mL in urine derivatized with TMPAH
Sim ions:
(Quant ion*) D5-Butalbital (*201,184,214): Butalbital (*196,181,138): Amobarbital (*169,184,225): Pentobarbital (*169,184,225): Secobarbital (*196,181,138): D5-Phenobarbital (*233,151,122): Phenobarbital (*232,146,117)

Analyte Details

Analyte Detail not available

1
Butalbital-d5

2
Butalbital
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3
Amobarbital
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4
Pentobarbital
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5
Secobarbital
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6
Phenobarbital-d5
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7
Phenobarbital
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