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uCATs by LCMS using Luna Omega 3um Polar C18 50x4.6mm

Sample Preparation Details

Condition 1 :0.2 mL Methanol
Condition 2 :0.2 mL 50 mM Ammonium acetate, pH 7
Load :Load 1.00 mL Sample diluted in Pretreated sample
Wash 1 :0.2 mL 50 mM Ammonium acetate, pH 7
Wash 2 :0.2 mL Acetonitrile / Isopropyl acohol (1:1)
Dry :3 minutes under high vacuum
Elution 1 :0.025 mL Water / Acetonitrile / Formic acid (85:10:5)
Elution 2 :0.025 mL Water / Acetonitrile / Formic acid (85:10:5)
Evaporate: :Evaporate to dryness
Notes :Pool eluents and dilute with 100 µL of 0.1% Formic acid in water

Application Detail (App ID: 24171)

Part No:00B-4760-E0
Dimensions:50 x 4.6 mm ID
Elution Type:Gradient
Elution A:Water with 0.1% formic acid
Elution B:Methanol with 0.1% formic acid
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 100 0
2 3 0 100

Flow Rate:0.7 mL/min
Col. Temp: 25 °C
Detection: Tandem Mass Spec (MS-MS) @ (22°C)
Detector Info: AB Sciex API 4000™

Analyte Details

Analyte Detail not available

1
Metanephrine
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2
Normetanephrine
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3
3-Methoxytyramine
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