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Chromatographic Separation of Isomeric GCDCA and GDCA on Kinetex 2.6µm Polar C18 100x2.1 Column

Application Detail (App ID: 24194)

Part No:00D-4759-AN
Dimensions:100 x 2.1 mm ID
Elution Type:Gradient
Elution A:2mM Ammonium acetate (pH 6.9)
Elution B:Methanol/Acetonitrile (50-50)
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 55 45
2 9 30 70
3 9.5 30 70
4 9.51 55 45
5 12 55 45

Flow Rate:400 mL/min
Col. Temp: 50 °C
Detection: Mass Spectrometer (MS) @ amu (50°C)
Detector Info: AB Sciex 4500 QTRAP®

Analyte Details

Analyte Detail not available

1
GCDCA

2
GDCA
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