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Applications
MeOH-2 Guar Gum Extraction Chromatography on Luna Omega 3u Polar C18
24445
Separation Mode: Reversed Phase
Reversed Phase
Polar C18
HPLC
Vitamin B Complex

Applications

MeOH-2 Guar Gum Extraction Chromatography on Luna Omega 3u Polar C18
Analytes
1Thiamine
Compound Name
Thiamin
CID
1130
Molecular Formula
C12H17N4OS+
Molecular Weight
265
No. Hydrogen Bond Acceptors
4
No. Hydrogen Bond Donors
2
Total Formal Charge
1
Details
LC Conditions (App ID: 24445)
Column:
Brand Name:
Part No:
Phase Name:
Polar C18
Elution Type:
Gradient
Mobile Phase:
A: 20 mM Sodium Phosphate (pH 3.5 with H3PO4)
B: Methanol
Gradient Profile:
Step No.Time(min)%A%B
101000
2154060
Flow Rate:
0.5 mL/min
Temperature:
22°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) @ 280 nm (22°C)

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