Applications

Cannabinoids from Human Urine Using Strata-X-C and Luna Omega 3µm Polar C18 100x3.0mm

Analytes

1AB-FUBINACA
2XLR-11 4-Hydroxypentyl metabolite
3AB-PINACA
4THC-OH
5THC-COOH
6APINACA (AKB-48) 5-Hydroxypentyl metabolite
7THC

Analyte not found

Details

LC Conditions (App ID: 24525)

Column:

Luna Omega 3 µm Polar C18 100 Å, LC Column 100 x 3.0 mm, Ea

Brand Name:

Luna Omega

Part No:

00D-4760-Y0

Phase Name:

Polar C18

Elution Type:

Gradient

Mobile Phase:

A: 0.1% formic acid

B: 0.1% formic acid/methanol

Gradient Profile:

Step No.	Time(min)	%A	%B
1	0	90	10
2	2	5	95
3	4	5	95
4	4.01	90	10
5	6	90	10

Flow Rate:

0.6 mL/min

Temperature:

25°C

Detection:

Tandem Mass Spec (MS-MS) (25°C)

Sample Preparation Details

Description: Strata-X-C 33 µm Polymeric Strong Cation, 30 mg / 3 mL, Tubes, 50/Pk
Part Number: 8B-S029-TBJ
Pre Treatment Note: Sample Prep Product: Strata-X-C, 30 mg/3 mL (Part No. 8B-S029-TBJ) Sample pre-treatment To 200uL Human urine sample add 100uL 0.1M ammonium acetate buffer (pH 4.0 ) in a glass test tube. Mix/vortex another 15 secs. Add 40uL b-Glucoronidase solution (100,000 units/mL from Campbell Science; Product # DR2102). Vortex 10-15 secs. Incubate for 2 hours in a shaker at 55o C to complete hydrolysis of the glucuronides. Add 400ul of 0.1% FA (formic acid) followed by vortexing for another 60 secs. SPE protocol Condition: 1 mL methanol Equilibrate: 1 mL water Load: Sample from pre-treatment step Wash 1: 1 mL water Wash2: 1 mL 40% methanol Dry: 3 to 4 mins at high vacuum (~10-15" of Hg) Elute: 2x200 uL (2 aliquots of 200 uL) of methanol Dry Down: Not required Direct injection: Eluted samples directly shot on the LC-MS-MS
Condition 1: Methanol
Condition 2: Water
Sample PreTreatment: Sample Prep Product: Strata-X-C, 30 mg/3 mL (Part No. 8B-S029-TBJ) Sample pre-treatment To 200uL Human urine sample add 100uL 0.1M ammonium acetate buffer (pH 4.0 ) in a glass test tube. Mix/vortex another 15 secs. Add 40uL b-Glucoronidase solution (100,000 units/mL from Campbell Science; Product # DR2102). Vortex 10-15 secs. Incubate for 2 hours in a shaker at 55o C to complete hydrolysis of the glucuronides. Add 400ul of 0.1% FA (formic acid) followed by vortexing for another 60 secs. SPE protocol Condition: 1 mL methanol Equilibrate: 1 mL water Load: Sample from pre-treatment step Wash 1: 1 mL water Wash2: 1 mL 40% methanol Dry: 3 to 4 mins at high vacuum (~10-15" of Hg) Elute: 2x200 uL (2 aliquots of 200 uL) of methanol Dry Down: Not required Direct injection: Eluted samples directly shot on the LC-MS-MS
Wash 1: Water
Wash 2: 40% methanol
Evaporation: Evaporate to dryness
1 Condition Methanol
2 Wash 40% methanol
1 Condition Methanol
2 Wash 40% methanol
1 Condition Methanol
2 Wash 40% methanol

Order Items Used in This Application

00D-4760-Y0

Luna Omega 3 µm Polar C18 100 Å, LC Column 100 x 3.0 mm, Ea

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Cannabinoids from Human Urine Using Strata-X-C and Luna Omega 3µm Polar C18 100x3.0mm

Order Items Used in This Application

00D-4760-Y0

Luna Omega 3 µm Polar C18 100 Å, LC Column 100 x 3.0 mm, Ea

Details

LC Conditions

Column:

Luna Omega 3 µm Polar C18 100 Å, LC Column 100 x 3.0 mm, Ea

Brand Name:

Luna Omega

Part No:

00D-4760-Y0

Phase Name:

Polar C18

Elution Type:

Gradient

Mobile Phase:

A: 0.1% formic acid
B: 0.1% formic acid/methanol

Gradient Profile:

Step No.	Time(min)	%A	%B
1	0	90	10
2	2	5	95
3	4	5	95
4	4.01	90	10
5	6	90	10

Flow Rate:

0.6 mL/min

Temperature:

25°C

Detection:

Tandem Mass Spec (MS-MS) (25°C)

LC Sample Preparation Details

Description:	Strata-X-C 33 µm Polymeric Strong Cation, 30 mg / 3 mL, Tubes, 50/Pk
Part Number:	8B-S029-TBJ
Phase Name:	X-C, Strong Cation Mixed Mode
Pre Treatment Note:	Sample Prep Product: Strata-X-C, 30 mg/3 mL (Part No. 8B-S029-TBJ) Sample pre-treatment To 200uL Human urine sample add 100uL 0.1M ammonium acetate buffer (pH 4.0 ) in a glass test tube. Mix/vortex another 15 secs. Add 40uL b-Glucoronidase solution (100,000 units/mL from Campbell Science; Product # DR2102). Vortex 10-15 secs. Incubate for 2 hours in a shaker at 55o C to complete hydrolysis of the glucuronides. Add 400ul of 0.1% FA (formic acid) followed by vortexing for another 60 secs. SPE protocol Condition: 1 mL methanol Equilibrate: 1 mL water Load: Sample from pre-treatment step Wash 1: 1 mL water Wash2: 1 mL 40% methanol Dry: 3 to 4 mins at high vacuum (~10-15" of Hg) Elute: 2x200 uL (2 aliquots of 200 uL) of methanol Dry Down: Not required Direct injection: Eluted samples directly shot on the LC-MS-MS
SPE Condition 1:	Methanol
SPE Condition 2:	Water
SPE Sample PreTreatment:	Sample Prep Product: Strata-X-C, 30 mg/3 mL (Part No. 8B-S029-TBJ) Sample pre-treatment To 200uL Human urine sample add 100uL 0.1M ammonium acetate buffer (pH 4.0 ) in a glass test tube. Mix/vortex another 15 secs. Add 40uL b-Glucoronidase solution (100,000 units/mL from Campbell Science; Product # DR2102). Vortex 10-15 secs. Incubate for 2 hours in a shaker at 55o C to complete hydrolysis of the glucuronides. Add 400ul of 0.1% FA (formic acid) followed by vortexing for another 60 secs. SPE protocol Condition: 1 mL methanol Equilibrate: 1 mL water Load: Sample from pre-treatment step Wash 1: 1 mL water Wash2: 1 mL 40% methanol Dry: 3 to 4 mins at high vacuum (~10-15" of Hg) Elute: 2x200 uL (2 aliquots of 200 uL) of methanol Dry Down: Not required Direct injection: Eluted samples directly shot on the LC-MS-MS
SPE Wash 1:	Water
SPE Wash 2:	40% methanol
SPE Evaporation:	Evaporate to dryness

Analytes

1. AB-FUBINACA

2. XLR-11 4-Hydroxypentyl metabolite

3. AB-PINACA

4. THC-OH

5. THC-COOH

6. APINACA (AKB-48) 5-Hydroxypentyl metabolite

7. THC

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