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Separation of Isobaric cannabinoids AB-PINACA and THC-OH on Luna Omega 3µm Polar C18 100x3.0mm

Sample Preparation Details

Condition 1 :1 mL Methanol at 1 mL/min
Condition 2 :1 mL Water at 1 mL/min
Load :Load 0.20 mL Sample diluted in 0.1% formic acid
Wash 1 :1 mL Water at 1 mL/min
Wash 2 :1 mL 40% methanol at 1 mL/min
Dry :3-4 mins at 10-15" of Hg
Elution 1 :0.2 mL methanol at 1 mL/min
Elution 2 :0.2 mL methanol at 1 mL/min
Evaporate: :Evaporate to dryness
Reconstitute :0 µL initial mobile phase
Notes :Sample Prep Product: Strata-X-C, 30 mg/3 mL (Part No. 8B-S029-TBJ) Sample pre-treatment To 200uL Human urine sample add 100uL 0.1M ammonium acetate buffer (pH 4.0 ) in a glass test tube. Mix/vortex another 15 secs. Add 40uL b-Glucoronidase solution (100,000 units/mL from Campbell Science; Product # DR2102). Vortex 10-15 secs. Incubate for 2 hours in a shaker at 55o C to complete hydrolysis of the glucuronides. Add 400ul of 0.1% FA (formic acid) followed by vortexing for another 60 secs. SPE protocol Condition: 1 mL methanol Equilibrate: 1 mL water Load: Sample from pre-treatment step Wash 1: 1 mL water Wash2: 1 mL 40% methanol Dry: 3 to 4 mins at high vacuum (~10-15" of Hg) Elute: 2x200 uL (2 aliquots of 200 uL) of methanol Dry Down: Not required Direct injection: Eluted samples directly shot on the LC-MS-MS

Application Detail (App ID: 24526)

Part No:00D-4760-Y0
Dimensions:100 x 3 mm ID
Elution Type:Gradient
Elution A:0.1% formic acid
Elution B:0.1% formic acid/methanol
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 90 10
2 2 5 95
3 4 5 95
4 4.01 90 10
5 6 90 10

Flow Rate:0.6 mL/min
Col. Temp: 25 °C
Detection: Tandem Mass Spec (MS-MS) @ (25°C)

Analyte Details

Analyte Detail not available

1
AB-PINACA

2
THC-OH
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