Linearity curve of norcocaine from Oral fluid on a Strata-X-C and Knx 2.6um, XB-C!8 50x4.6 column

Application Detail (App ID: 24531)

Part No:00B-4496-E0
Dimensions:50 x 4.6 mm ID
Elution Type:Gradient
Elution A:0.1% Formic Acid in DI H2O
Elution B:0.1% Formic Acid in ACN
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 90 10
2 3 60 40
3 3.5 60 40
4 3.51 90 10
5 6 90 10

Flow Rate:1000 µL/min
Col. Temp: ambient
Detection: Tandem Mass Spec (MS-MS) @ (22°C)
Detector Info: Instrument: Agilent® 1260
MS/MS Instrument: AB SCIEX Triple Quad 5000; ESI Source (+ve ionization)
Note:Sample pre-treatment
1ml human oral fluid was collected on cellulose pad of the applicator tip provided by the Intercept® i2
oral fluid device (OFC) . Saturated pad was placed into
transport tube containing buffer solution and allowed to
sit overnight. Centrifuge at 600g for 15mins to collect
SPE cartridge: Strata-X-C, 30 mg 96-Well Plate
Part No. 8E-S029-TGB
Step Procedure
Condition: 1 mL Methanol
Equilibrate: 1 mL DI Water
Load: Combine 0.5mL of pretreated sample spiked with internal standards and 1 mL 1% formic acid, mix/vortex 10-15 secs and load in Strata-X-C well plate.
Weak Wash: 1 mL DI Water
Strong Wash: 1 mL 50:50 Acetone/Water
Dry Down: 5 minutes at maximum vacuum (15" Hg or higher)
Elute: 2 x 500 µL Methanol/Acetonitrile/30% Ammonium Hydroxide (5:5:2)
Dry Down: Evaporate to dryness under gentle nitrogen and 45-50°C.
Reconstitute: 200 µL initial mobile phase

Analyte Details

Analyte Detail not available