| 1 Intercept-i2 Oral Fluid devise |
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Applications
Q1 Scan showing sample cleanliness of Oral fluid SPE from ntercept buffer using Starta-X-C cartridge
24536
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Applications
Q1 Scan showing sample cleanliness of Oral fluid SPE from ntercept buffer using Starta-X-C cartridge
Analytes
Details
LC Conditions (App ID: 24536)
Column: | ||||||||||||||||||||||||
Brand Name: Kinetex | ||||||||||||||||||||||||
Part No: | ||||||||||||||||||||||||
Phase Name: C18 | ||||||||||||||||||||||||
Elution Type: Gradient | ||||||||||||||||||||||||
Mobile Phase: A: 0.1% formic aid in water B: 0.1% formic acid in MeOH | ||||||||||||||||||||||||
Gradient Profile:
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Flow Rate: 500 µL/min | ||||||||||||||||||||||||
Temperature: 22°C | ||||||||||||||||||||||||
Detection: Tandem Mass Spec (MS-MS) (22°C) | ||||||||||||||||||||||||
Detector Info: SCIEX 4000 QTRAP® | ||||||||||||||||||||||||
Sample Note: SPE Product: Strata-X-CW, 30 mg/3 mL cartridge
Part# 8E-S035-TBJ
Sample pre-treatment
Transfer 1 mL oral fluid collected on an applicator tip in its preservative buffer (Intercept). Leave it for 2 hours followed by centrifugation for 15 mins at 600g. Remove 0.5 mL of supernatant to combine with 1 mL 1% formic acid, mix/vortex 5-10 secs to load on Strata X-CW 30mg/3 mL cartridge.
Condition: 1 mL Methanol
Equilibrate: 1 mL DI Water
Load: Combine 0.5mL of pretreated sample with 1 mL 1% formic acid, mix/vortex 5-10 sec and load
Wash 1: 1 mL 1% Formic acid/DI Water
Wash 2: 1 mL DI Water
Dry Down: 5-6 minutes at maximum vacuum (20" Hg or higher)
Elute: 2 x 500 µL methylene chloride/Isopropanol/30% Ammonium Hydroxide (80:18:2)
Dry Down: Evaporate to dryness under gentle nitrogen and 45-50°C.
Reconstitute: With 167 uL initial mobile phase
SPE Product: CEREX OFX 3mg
Part# 633-003I NBE, 96 well NBE plate 3mg/1Ea
Sample pre-treatment
Transfer 1 mL oral fluid collected on an applicator tip in its preservative buffer (Intercept). Leave it for 2 hours followed by centrifugation for 15 mins at 600g. Remove 0.5 mL of supernatant to combine with 0.3 mL 0.1M HCl, mix/vortex 5-10 secs to load on SPEware cartridge
Condition: 500 uL methanol
Equilibrate: 500 uL water
Load: Sample from pre-treatment step
Wash1: 700 uL 0.1M HCl
Wash2: 2x0.8 mL D.I. water
Dry: 10 mins under 45050 psi
Elution: 300 uL of Dichloromethane:Isopropanol: 30%ammonium hydroxide (80/18/2)
Drydown: Evaporate to dryness under gentle nitrogen and 45-50°C.
Reconstitute:167 µL initial mobile phase |
Order Items Used in This Application
00B-4462-Y0
Kinetex 2.6 µm C18 100 Å, LC Column 50 x 3 mm, Ea
Qty:
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Intercept-i2 Oral Fluid devise
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