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Q1 Scan showing sample cleanliness of Oral fluid SPE from ntercept buffer using Starta-X-C cartridge

Application Detail (App ID: 24536)

Part No:00B-4462-Y0
Dimensions:50 x 3 mm ID
Elution Type:Gradient
Elution A:0.1% formic aid in water
Elution B:0.1% formic acid in MeOH
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 90 10
2 4 5 95
3 5.5 5 95
4 5.51 90 10
5 7 90 10

Flow Rate:500 µL/min
Col. Temp: ambient
Detection: Tandem Mass Spec (MS-MS) @ (22°C)
Detector Info: AB Sciex 4000 QTRAP®
Note:SPE Product: Strata-X-CW, 30 mg/3 mL cartridge
Part# 8E-S035-TBJ
Sample pre-treatment
Transfer 1 mL oral fluid collected on an applicator tip in its preservative buffer (Intercept). Leave it for 2 hours followed by centrifugation for 15 mins at 600g. Remove 0.5 mL of supernatant to combine with 1 mL 1% formic acid, mix/vortex 5-10 secs to load on Strata X-CW  30mg/3 mL cartridge.
Condition: 1 mL Methanol
Equilibrate: 1 mL DI Water
Load: Combine 0.5mL of pretreated sample with 1 mL 1% formic acid, mix/vortex 5-10 sec and load
Wash 1: 1 mL 1% Formic acid/DI Water
Wash 2: 1 mL DI Water
Dry Down: 5-6 minutes at maximum vacuum (20" Hg or higher)
Elute: 2 x 500 µL methylene chloride/Isopropanol/30% Ammonium Hydroxide (80:18:2)
Dry Down: Evaporate to dryness under gentle nitrogen and 45-50°C.
Reconstitute: With 167 uL initial mobile phase

SPE Product: CEREX OFX 3mg
Part# 633-003I NBE, 96 well NBE plate 3mg/1Ea
Sample pre-treatment
Transfer 1 mL oral fluid collected on an applicator tip in its preservative buffer (Intercept). Leave it for 2 hours followed by centrifugation for 15 mins at 600g. Remove 0.5 mL of supernatant to combine with 0.3 mL 0.1M HCl, mix/vortex 5-10 secs to load on SPEware cartridge
Condition: 500 uL methanol
Equilibrate: 500 uL water
Load: Sample from pre-treatment step
Wash1: 700 uL 0.1M HCl
Wash2: 2x0.8 mL D.I. water
Dry: 10 mins under 45050 psi
Elution: 300 uL of Dichloromethane:Isopropanol: 30%ammonium hydroxide (80/18/2)
Drydown: Evaporate to dryness under gentle nitrogen and 45-50°C.
Reconstitute:167 µL initial mobile phase


Analyte Details

Analyte Detail not available

1
Intercept-i2 Oral Fluid devise